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Figure 7


Fig. 7. Spatial regulation of Src activity by membrane cholesterol and S1P signalling in PDGF-stimulated cells. NIH 3T3 were transfected with an avian Src construct, synchronised in G0 by serum starvation, treated or not with indicated agent and stimulated or not (NS) for 10 minutes with PDGF as shown. Src was detected with EC10 antibody (red), active Src with anti-pY416 antibody (green) and actin with fluorescent Phalloidin (blue). Left panels: merged imaged of Src and active Src fluorescence. Middle panels: actin. Right panels: merged images of Src, active Src and actin. Pools of active Src at the plasma membrane and associated actinic dorsal ruffles are indicated by arrows.





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