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Figure 2


Fig. 2. (A,B) Time course of TRPL channel relocalization to the rhabdomere after (A) stage 1 and (B) stage 2 of light-induced translocation. (A) Wild-type flies were light-exposed for 2 hours to induce translocation to the stalk membrane, and then dark-incubated for increasing durations. Full recovery of TRPL localization to the rhabdomere required 6 hours of dark-incubation. Shown are representative ommatidia from multiple retinal sections taken from ten eyes of eight flies (Light, 2hrs), ten eyes of eight flies (Dark, 4hrs), ten eyes of seven flies (Dark, 6hrs). (B) Wild-type flies were light-exposed for 10 hours to induce translocation to the basolateral membrane, and then dark-incubated for increasing durations. After 6 hours of dark-incubation, TRPL gradually appeared in the stalk membrane in some photoreceptor cells. Full recovery of TRPL localization to the rhabdomeres was seen after 10 hours of darkincubation. For all of the above, wild-type retinal cross-sections were immunostained for TRPL. Shown are representative ommatidia from multiple retinal sections taken from five eyes of three flies (Light, 10hrs), five eyes of four flies (Dark, 6hrs), six eyes of six flies (Dark, 10hrs).





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