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Figure 1


Fig. 1. Direct co-culture of AT-SVF cells with primary myoblasts. Fresh inguinal AT-SVF cells from GFP mice were co-cultured with wild-type primary myoblasts for three days in GM and then switched to DM. Myogenic differentiation was revealed after one week by staining with an anti Troponin-T antibody (TnT). Several GFP cells (green) that have been incorporated into multinucleated myotubes expressing TnT (red) are visible. The arrow indicates a double GFP/TnT-positive mononucleated cell, shown in the inset at larger magnification, suggesting that AT-derived cells can differentiate into skeletal muscle in the absence of cell fusion with skeletal myotubes. (A) Fluorescence image of GFP positive cells, (B) fluorescence image of TnT-positive cells, (C) overlay of A and B; Hoechst staining was used to visualize nuclei (blue). Magnification, 20x. Bar, 100 µm.





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