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Figure 2


Fig. 2. Cell-autonomous myogenic differentiation of AT-SVF cells. Fresh inguinal AT-SVF cells were plated on fibronectin-coated transwell filters floating over a layer of primary myoblasts (A-C) or simply on a fibronectin-coated tissue-culture dish (D,E). Cultures were maintained for three days in proliferation medium and then switched to differentiation medium. Myogenic differentiation was revealed after 1 week by staining with an anti TnT antibody (red). (A,B) The image shows one of the several clusters of skeletal myotubes found on a transwell filter in a typical experiment. (C) RTPCR analysis for the indicated skeletal muscle markers of inguinal AT-SFV cells. AT, fresh, uncultured AT-SVF cells; AT+PM, AT-SVF cells cultured on transwell filters in the presence of primary myoblasts. Differentiating primary myoblasts (PM) were used as positive controls. (D,E) Spontaneous myogenic differentiation of ATSVF cells. (A,D) Fluorescence image of TnT positive cells; (B,E) merge with Hoechst to visualize nuclei (blue). (A,B) Magnification, 10x. Bar, 200 µm. (D,E) Magnification, 20x. Bar, 100 µm.





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