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Figure 3


Fig. 3. Myogenic conversion of AT-MSCs induced by primary myoblasts requires cell contact with myogenic cells. GFP-positive AT-MSCs were either seeded in the same dish together with primary myoblasts (top panels) or plated on 0.4 µm porous transwell filters floating on a layer of primary myoblasts (bottom panels). Myogenic differentiation was revealed by TnT staining (red) after 1 week in DM. Hoechst was used to visualize nuclei (blue). Double GFP/TnT-positive cells indicating myogenic differentiation of ATMSCs are observed only when contact between the two cell types is allowed (A-C). (A,D) Fluorescence image of GFPpositive cells; (B,E) fluorescence image of TnT-positive cells; (C,F) overlay of GFP, TnT and Hoechst staining. Magnification, 20x. Bars, 100 µm.





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