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Figure 4


Fig. 4. Spontaneously differentiating AT-SVF myogenic cells express satellite-cell-specific markers. (A) Transmission light image of a typical AT-derived group of proliferating myogenic cells, 5 days after plating in GM. (B) The same group of cells shown in A after 24 hours in DM, when myotubes start to form (magnification, 20x; Bar, 50 µm). (C,D) Immunofluorescence staining with an anti-Pax7 antibody of proliferating myogenic cells. To highlight the specificity of the Pax7 staining, we chose an image field where myogenic cells groups were close to morphologically different, non-myogenic cells. Small nuclei of myogenic cells express Pax7 while bigger nuclei of adjacent, nonmyogenic cells, are negative for Pax7. (C) Fluorescence image of Pax7-positive cells (red). (D) merge with Hoechst (blue) to visualize nuclei. Magnification, 40x. Bar, 25 µm. (E) RT-PCR analysis for the indicated markers of isolated myogenic clones. pPM, proliferating, skeletal-muscle-derived, primary myoblasts; pATM, proliferating AT-derived myogenic clones; AT, fresh, uncultured AT-SVF cells.





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