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Fig. 5. AT-SVF cells participate in skeletal muscle regeneration. ATSVF cells from GFP mice were injected into the adductor muscle of GFP-negative syngenic mice were ischemia was induced by femoral artery removal. Engrafted GFP-expressing cells in the injected muscle were visualized by an anti-GFP antibody (green) 7 days after injection. A wide region of GFP positive fibers are present in muscle sections from treated hind limbs (B) while no GFP staining is observed in control sections from PBS-injected limbs (A). Nuclei are visualized by Hoechst (blue). Magnification, 40x. Bar, 50 µm.