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Figure 6


Fig. 6. Transplantation of wild-type AT-SVF cells rescues dystrophin expression in mdx mice. Immunostaining of adductor muscles transverse sections with an antibody against the C-terminal portion of dystrophin. Fresh AT-SVF cells were injected into the left adductor muscle of mdx mice. PBS injected age-matched mdx mice were used as controls. Staining for dystrophin (green) was performed 21 days after injection. Nuclei are visualized by Hoechst (blue). Dystrophin expression is totally absent in PBS-injected muscles while clusters of dystrophin-positive fibers are with both centrally and peripherally located nuclei in mice injected with AT-SVF cells. (A) Wild-type uninjected; (B) mdx injected with PBS; (C) mdx injected with AT-SVF cells. Magnification, 40x. Bar, 50 µm.





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