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Figure 1


Fig. 1. A directed two-hybrid screen for MTM interactions. (A) Schematic diagram indicating domain structure of the myotubularin family. Point mutations used in this study are indicated by asterisks. (B) Example of a yeast two-hybrid screen for MTMR4 interactions with other MTM family members. Yeast cells were cotransfected with bait-MTMR4 and all different preys (AC). On the selective QDO plate (right), growth (interaction) was observed with MTMR3 and MTMR4 in the prey vectors (see scheme on the left). A catalytically inactive mutant of MTMR3 (C413S) retains binding to MTMR4. As a control, the same co-transformations were plated onto SD-leu/trp (centre).





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