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Figure 2


Fig. 2. Co-immunoprecipitation of human myotubularin family members. HeLa cells were transfected with HAMTMR3, HA-MTMR4 or HAMTMR9, and different EGFP-tagged myotubularins as indicated. EGFP proteins were immunoprecipitated from cell lysates and processed for western blotting. Corresponding membranes were blotted with an anti-HA antibody (top panels) and confirmed interactions identified in the two-hybrid screen. (A) MTMR3 and MTMR4 interact with each other and with themselves. A truncated form of MTMR3 (1-821) lacking the Cterminal coiled-coil domain preserves this interaction. (B) MTMR8 interacts with MTMR9 (arrow, top panel). Membranes were re-probed with anti-EGFP (bottom panels) and lysates with an anti-HA (middle panels) showing the efficiency of EGFP immunoprecipitation and HA-protein expression levels, respectively.





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