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Fig. 4. MTMR4 localizes to endosomes. HeLa cells were transfected with EGFP-MTMR4 and co-stained after fixation with anti-EEA1 or anti-Hrs respectively (A,B). (C) AlexaFluor594-Transferrin was internalised into transfected cells during a 30 minute incubation. (D) Transfected cells were treated with wortmannin for 1 hour before fixation. Bar, 10 µm.
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