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Figure 6


Fig. 6. Localisation and influence upon the endosomal PtdIns3P pool following mutant MTMR4 expression. HeLa cells were transfected with HA-MTMR4-C407S (catalytically inactive, A-C and G-I) or HA-MTMR4-C1169S (FYVE domain mutant, D-F and J-L) fixed and stained with anti-HA and EEA1 antibodies (A-F) or anti-HA and biotinyl-GST-2xFYVE probe (G-L) as described in Materials and Methods. Bar, 10 µm.





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