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Figure 5


Fig. 5. Recognition of SGLT1 on the surface of intact cells by a D-glucose primed AFM tip. (a) The effect of phlorizin and the antibody PAN32 in the presence of Na+ (KRH-NaCl) on the binding probabilities of D-glucose tip on G6D3 (gray) and CHO cells (black). (b) Probability density function (pdf) giving the distribution of the unbinding force fu required to disrupt single glucose-receptor interaction (n=1000). (c) Binding probabilities of D-glucose tip on G6D3 cells in the presence and in the absence of Na+ (KRH-NaCl) (gray) and KRH-NMG (sodium replaced by Nglucosamine) (blue), respectively. Values are mean ± s.e.m., n=2000-4000. *P<0.05 and ***P<0.005 compared with levels in the control group (unblocked).





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