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Figure 1


Fig. 1. TbDLP is required for Bax-induced mitochondrial fission. (A) Growth curve of the inducible (–Tet, +Tet) T. brucei TbDLP-RNAi cell line. RNAi was confirmed by northern blot (middle panel). Right panel: Immunofluorescence of induced cells using an inner mitochondrial membrane-specific F1-ATPase antiserum. The fraction of cells showing a fragmented mitochondrion is indicated. Bottom panel: Kinetics of TbDLP ablation tested by co-expression of a HAtagged TbDLP (TbDLP-HA). The tagged protein was detected on immunoblots using anti HA-tag antiserum Y-11 (Santa Cruz Biotechnology). Hours of induction are indicated at the top. The lower half of the panel shows the Coomassie-stained tubulin region and serves as a loading control. (B) Same as A, but data are from a T. brucei cell line allowing inducible expression of human Bax (Crausaz-Esseiva et al., 2004). Bax expression was verified by immunoblot. (C) Same as A, but data are from a cell line allowing inducible expression of Bax and RNAi of TbDLP at the same time. The growth curve for the Bax-expressing cell line, as in B, is shown in grey for comparison. Standard errors (n=3-7) are indicated. Bar, 25 µm.





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