spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Lack of TbDLP leads to a specific arrest of cytokinesis. (A) Analysis of nuclei and kDNA configurations of DAPI-stained cells during induction of TbDLP-RNAi. The graph indicates the percentages of cells containing the indicated numbers of nuclei and kDNAs (1K1N, 2K1N, 2K2N and others; n>1000 cells). Percentages of NKKN-cells, a subgroup of 2K2N cells where the two kDNAs are localized between the two nuclei, are also indicated. (B) NKKN-cells have a single mitochondrion. 3D-reconstruction from optical sections obtained by confocal microscopy of an anti-Hsp60 (green) and DAPI co-stained (blue) NKKN cell from the induced TbDLP-RNAi cell line. (C) NKKN cells have enlarged FPs. Visualization of FPs was done as in Fig. 2A. (D) Same as B, but staining was with the antibody L3B2 (green), which recognizes the flagellar attachment zone (FAZ) (Kohl et al., 1999). Bars, 2.5 µm.





Right arrow Return to article