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Figure 2


Fig. 2. KCNQ4 expression in control and hypothyroid rats. (A) Double immunohistochemistry of prestin (red) and KCNQ4 (green) in OHCs of control and hypothyroid (hypo) rats at P21. (B) Using northern blotting, both, the ~3.9 kb and ~3.8 kb Kcnq4 transcripts (filled arrows), are reduced in the cochleae of P12 hypothyroid rats (hypo) compared with controls (con), detected with a Kcnq4-specific antisense riboprobe (as). Sense control shows no signal. Blots were probed with a cyclophilin-antisense probe (open arrow) as a housekeeping gene. (C) Reduced Kcnq4 mRNA levels in the cochlea of P12 hypothyroid rats (hypo) compared with controls (con) is also noted using semi-quantitative RT-PCR. The housekeeping gene cyclophilin was used as a control. (D) Localization of Kcnq4 mRNA in control and hypothyroid (hypo) rat cochleae at P18 using in situ hybridization. Note the strong signals in OHCs and a weaker signal in IHC of controls, whereas no signals are detectable in hair cells of hypothyroid rats. Bars, 10 µm (A); 20 µm (D).





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