JCS03030 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Rat PMCA1xb and PMCA2wa aa sequence alignment. Aa sequence alignment using MegAlign (DNAstar, Madison) of rat PMCA1xb (1220 aa) and PMCA2wb (1243 aa), showing more than 85% identity. Their transmembrane domains are shown in pink, their phospholipids binding sites are shown in yellow and their respective A-splice x and w cassettes are highlighted in green and show a divergence in the aa composition. Substitutions of four aa residues in the PMCA1-encoding construct are highlighted with pink discs.

• Movie 1 -

  Movie 1. PMCA2xb isoform is targeted exclusively basolaterally in MDCK-I. 3D-reconstruction of a confocal stack of images of polarized MDKC-I cells expressing EGFP-tagged PMCA2xb isoform (green); tight-junction protein ZO-1 is labeled red.

• Movie 2 -

  Movie 2. PMCA2wa isoform is targeted exclusively apically in MDCK-I. 3D-reconstruction of a confocal stack of images of polarized MDKC-I cells expressing EGFP-tagged PMCA2wa isoform (green). Tight-junction protein ZO-1 is labeled red.