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Figure 1


Fig. 1. PMCA1 and PMCA2 alternative splicing. (A) PMCA secondary structure. The positions of the phospholipid binding region (G) and the location of the deafwaddler mouse Gly283Ser mutation (dfw G>S) in PMCA2 are shown. PM, plasma membrane. (B,C) Several PMCA1 and PMCA2 variants are encoded by differentially spliced mRNA. PMCA2 isoforms w, y, x and z are generated by alternative splicing at the A-splice site located in the first cytoplasmic loop. Isoforms differing in their C-termini are generated by alternative splicing at the C-splice site. (B) Aa sequences of PMCA2 exons 1, 2 and 3 of the A-splice site, and of the a-, b- and c-tails generated by alternative splicing at the C-splice site. (C) Aa sequence of the constitutively spliced exon in the PMCA1 A-splice site, and of the a-, b-, c-, d- and e-tails generated by alternative splicing at the C-splice site. Residues Glu-Thr-Ser-Leu (ETSL, underlined in B and C) in the most C-terminal sequence of the b-tail form a PDZ-domain-interacting motif. Aa numbers are according to GenBank PMCA2wb sequence (1243 aa, GenBank accession number P11506), PMCA1xd sequence (1258 aa, GenBank accession number P11505). (D) Aa sequence alignment of PMCA1 and PMCA2 b-tails.





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