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Figure 4


Fig. 4. PMCA1xb basolateral localization in HCs requires the b-tail. (A) Secondary structure of PMC1xb with a single insert (13 aa) in the A-splice site (1, yellow) and the b-tail (b, purple). (B,C) Surface scans (plane 2 in D) and orthogonal views (plane 1 in D) of stacks of confocal images of HCs transfected with wild-type EGFP-tagged PMCA1xb (B) and PMCA1xb deleted of the b-tail aa sequence (C); green (GFP), red (actin), yellow (merged). Small panels show high-resolution cross-sectional (plane 3 drawn in D) scans in merged channels. n, number of examined transfected hair cells. Bars, 5 µm.





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