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Figure 8


Fig. 8. The basolateral targeting of PMCA2 is not mediated by adaptin AP1B, and the targeting signal is transposable to other PM proteins. (A-C) High-resolution surface confocal scans of transfected polarized LLC-PK1 cells, taken around the junctional area (upper panels) and orthogonal views of stacks of confocal images (lower panels). (A) PMCA2xb (green) is exclusively basolaterally targeted in LLC-PK1. (B) IL-chain (Tac) is delivered predominantly apically in LLC-PK1 cells, with a visible basolateral component. Under the influence of the b-tail, the chimeric protein Tac, fused to the b-tail (Tac-2b), is predominantly delivered to the basolateral PM (C). The mutations in the b-tail Leu1153Ala and Ile1154Ala, inactivate the signal in the b-tail and the product of construct Tac-2b-LI1153-1154AA is targeted predominantly apically (D), similarly to the Tac alone. Bars, 10 µm.





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