spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. The size of the insert of the A-splice site determines the apical targeting of PMCA2. (A) Schematic representation of the wild-type PMCA2wa isoform (wt-2wa) used to study the targeting properties of isoforms with various insert sizes. Aa sequence shows the three consecutive exons forming the w-insert of the A-splice site. (B-H) Confocal scans of the hair bundles (plane 2 in I; actin in red) of HCs expressing mutated variants of PMCA2wa (green) are shown in green and merged channels. High-resolution cross-sectional scans of the basolateral PM (plane 3 in I) are shown in the green channel. Mutations generated for each variant are shown in red on the aa sequence of the w-insert. (J) Summary of our results. Only isoforms with inserts of at least 31 aa in the A-splice site are targeted exclusively apically. a, a-tail; AP, apical PM localization; BL, basolateral PM localization. Bars, 5 µm.





Right arrow Return to article