QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Kit-targeting scheme showing a partial map of a mouse genomic DNA fragment containing Kit exon 1 of wild-type allele (A) and the resultant targeted alleles, Kit^W-lacZ allele (B) and Kit^W-FKB (C). The BamHI and Kpn sites mark the 5'and 3' ends of the targeting vector, respectively. (D) Multiplex PCR analysis of DNA isolated from Kit^+/+ (lane 1), Kit^W-lacZ/+ lane 2) and Kit^{W-lacZ/W-lacZ} (lane 3) cell lines. The 1.4 and 1.8 kb fragments represent the wild-type and WlacZ alleles respectively. (E) Southern blot analysis of EcoR1-digested DNA isolated from Kit^{W-lacZ/W-lacZ} (lane 1), Kit^W-lacZ/+ (lane 2), Kit^+/+ (lane 3) and Kit^W-lacZ/W-FKB (lane 4) targeted ES cell clones. The 9.1, 6.4 and 2.4 EcoRI restriction fragments represent the wild-type, Kit^W-lacZ and Kit^W-FKB alleles respectively. (F) Flow cytometric analysis of Kit^+/+, Kit^W-lacZ/+ and Kit^W-lacZ/W-FKB cell lines using a TC-conjugated anti-mouse KIT monoclonal antibody showing no KIT expression in the Kit^W-lacZ/W-FKB cell line. Restriction enzymes: B, BamHI; EI, EcoRI; EV, EcoRV; K, KpnI.

Return to article