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Fig. 2. (A) Morphology of ES cells growing in the presence of 100 U/ml LIF. (B) Growth rate of the different cell lines during routine passage expressed as the total number of cells present 2 days after plating 1x10⁶ cells. The solid bars within the individual scatter represent the median of six independent passages. (C) Total number of colonies produced from Kit^+/+ (+/+), Kit^W-lacZ/+ (+/-), Kit^{W-lacZ/W-lacZ} (-/-) and Kit^W-lacZ/W-FKB (-/FKB) ES cells 5 days after plating at low density in varying concentrations of LIF. Colonies were scored as stem cells (SC), differentiated (DIFF) or mixed (MIX) according to their morphology and AP-staining profile. The results shown are representative examples of several independently derived clones that were all assayed three times in duplicate. (D) The combined number of mixed and differentiated colonies produced from Kit^+/+ (+/+), Kit^W-lacZ/+ (+/-), Kit^{W-lacZ/W-lacZ} (-/-) and Kit^W-lacZ/W-FKB (-/FKB) ES cells 5 days after plating at low density in 100 U/ml LIF. Values shown represent the mean (± s.e.m.) of four independent experiments performed in duplicate. (E) Number of colonies produced from wild-type ES cells after plating at low density in varying concentrations of LIF in the presence (+) or absence (-) of the monoclonal anti-KIT antibody, ACK2.

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