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Figure 4


Fig. 4. Redistribution of hOR17-4 in human spermatozoa upon bourgeonal application. (A) Subcellular localization of hOR17-4 is changed upon bourgeonal stimulation of human spermatozoa. An antibody against hOR17-4 raised in mouse labeled the midpiece of non-treated human spermatozoa (0), some cells show a ring-like staining around the head; after bourgeonal stimulation (20 min) staining is strongly decreased in the midpiece. Treatment with undecanal blocks hOR17-4 redistribution, similar to ß-arrestin2. Bar, 10 µm. (B) Western blot analysis of human sperm with the anti-hOR17-4 antibody shows that the hOR17-4 protein can be detected at ~40 kDa in the total cell lysate, but does not translocate to the nucleus in response to bourgeonal stimulation. (C) Control western blots showing the specificity of the antibody. Equal amounts of hOR17-4-GFP-transfected HEK293 cells and mock-transfected cells were probed with anti-hOR17-4 and anti-GFP antibody.





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