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Figure 2


Fig. 2. Co-immunoprecipitation of ICAM-5 with {alpha}-actinin. (A) {alpha}-actinin was immunoprecipitated from Paju-ICAM-5 cell lysates with a specific anti-{alpha}-actinin antibody and the precipitates were subjected to SDS-PAGE and immunoblotted with anti-ICAM-5 antibody to detect possible co-immunoprecipitation of ICAM-5. The successful precipitation of {alpha}-actinin was ensured by immunoblotting with anti-{alpha}-actinin antibody (not shown). (B) ICAM-5 was immunoprecipitated from rat brain homogenates and the precipitates were run on SDS-PAGE and immunoblotted for ICAM-5 and after stripping for {alpha}-actinin.





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