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Figure 6


Fig. 6. Effect of ICAM-5 contructs on neuritic outgrowth. (A) The Paju-ICAM-5 (a-d, I-IV), Paju-ICAM-5-TM (e-h) and Paju-ICAM-5-KK/AA cells (i-l) were activated by 100 nM PdBu to induce neurite outgrowth. The cells were triple-stained for {alpha}-actinin (green), actin (red), ICAM-5 (magenta), and visualized by confocal microscopy. Concentrations of ICAM-5, {alpha}-actinin and F-actin at cell-anchorage sites (a-d) and growth cones of neurites (I-IV) in Paju-ICAM-5 cells were seen. Bars, 20 µm. (B) Histogram displaying neurite outgrowth in PdBu-activated Paju-ICAM-5, Paju-ICAM-5-TM and Paju-ICAM-5-KK/AA cells (neurite length presented as micrometers). The differences between the neuritic length in Paju-ICAM-5 cells compared with Paju-ICAM-5-TM and Paju-ICAM-5-KK/AA cells are statistically significant (P<0.001). Standard deviations are shown.





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