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Figure 2


Fig. 2. Folding of the wild-type GHR. Transiently transfected ts20 cells were pulse-labelled for 10 minutes and chased for the indicated times. (A) Postnuclear lysates were split in equal amounts and either immunoprecipitated with anti-GHR-T (left panel), or isolated with btGH and streptavidin beads (right panel). Samples were subjected to reducing SDS-PAGE. p, 110-kDa precursor form; m, 130-kDa mature form. (B) After immunoprecipitation, samples were treated with EndoH (+) or mock treated (-). All samples were immunoprecipitated with anti-GHR-T and subjected to reducing SDS-PAGE. (C) Cells were pre-incubated with MG132 for 1 hour and subsequently treated with MG132 during pulse and chase periods (+), or mock treated (-). Samples were immunoprecipitated with anti-GHR-T and subjected to reducing SDS-PAGE. (D) After immunoprecipitation, samples were subjected to reducing (+) and non-reducing (-) SDS-PAGE. Relative molecular mass standards are indicated.





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