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Figure 5


Fig. 5. Dimerisation of the GHR mutations. Full-length (FL) wild-type (WT) and mutant GHRs were transiently transfected in ts20 cells, either together with GHR(1-369; HA-6xHis-Myc) (369-HA) bearing the different mutations or with empty vector (EV). 369-HA receptors were also co-transfected with EV. After lysis, full-length receptors were immunoprecipitated with anti-GHR-C and separated by reducing SDS-PAGE. (A) Immunoblotting of lysates with anti-HA antibody. Transfection combinations (+) are indicated. Mutant GHRs as indicated in Fig. 3. (B) Immunoblotting of anti-GHR-C immunoprecipitates with anti-HA antibody. (C) Efficiency of immunoprecipitation was determined by reprobing the blot shown in B with anti-GHR antibody Mab5. M, mix of lysate from ts20 cells transiently transfected with full-length GHR (mutants) and empty vector with lysate from ts20 cells transiently transfected with 369-HA GHR (mutants) and empty vector; m, mature GHR; p, precursor GHR; IP, immunoprecipitation; WB, immunoblotting. Relative molecular mass standards are shown on the left.





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