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Figure 6


Fig. 6. Cell-surface appearance of the mutant receptors. Cells transiently expressing empty vector, wtGHR (WT) or the GHR mutants were surface stained with monoclonal antibody Mab5 and subsequently with Cy5-conjugated goat-anti-mouse IgG. Living cells were gated and analysed by flow cytometry. WtGHR and the mutants are indicated with open histograms and shown as an overlay with the negative control, empty vector transfected cells (solid histogram). Receptor species are indicated as in Fig. 3. The data shown are representative of three independent experiments.





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