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Figure 7


Fig. 7. Signalling capacities of the mutant receptors. Transiently transfected ts20 cells expressing wtGHR (WT), GHR(C108S) or GHR(C108A-C122A) (C) were treated for 15 minutes with 8 nM btGH (GH) or btGHA (GHA) at permissive temperature (30°C) and lysed. (A) Immunoblotting of lysates with anti-GHR-C. After lysis, btGH(A)-GHR complexes were isolated with streptavidin beads and subjected to reducing SDS-PAGE. (B) Immunoblotting of complexes with anti-GHR-C. (C) The same blot as in B immunoblotted with anti-phosphotyrosine (anti-PY) antibody. m, mature GHR; p, precursor GHR; IP, immunoprecipitation; WB, immunoblotting. Relative molecular mass standards are shown on the left.





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