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Figure 1


Fig. 1. Cellular and subcellular distribution of Kir6.1- and SUR2A-immunoreactivities in the human gastric mucosa. Antibodies against SUR2A (a and b, red; e, green; g, yellow when colocalized with ZO-1) or against Kir6.1 (c and d, green or yellow - when colocalized with E-cadherin) yield a distinct dot-like or string-like staining pattern of the plasma membrane. (a) SUR2A immunoreactivity is completely abolished by preabsorption with its cognate recombinant protein (inset). (b) Localization of the gastric anion exchanger AE2 (green), a marker of parietal cell basolateral plasma membrane, and SUR2A does not overlap. Red dots correspond to the SUR2A protein (both insets); they are restricted to the apical edge of the basolateral plasma membrane. (c,d) Adherens points can be visualized by staining for E-cadherin (red). The Kir6.1 protein (green) is restricted to the apical edge of the basolateral surface, presumably owing to the close spacing of tight junctions and adherens points at the lumenal border of the cells (d, arrows). (e,f) By contrast, SUR2A (green) colocalizes perfectly with the tight junction marker ZO-1 (red). (g) Merged image of e and f, highlighting the entire distribution of the tight junctions (g). Bars, 30 µm.





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