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Figure 6


Fig. 6. (a) Tight junctions between urothelial facet cells are devoid of Kir6.1 immunoreactivity. (c,d) The morphology of the lumenal surface of the rat urinary bladder is visualized by electronically intensifying its weak autofluorescence. The lumenal surface is covered by the urothelium, a specialized epithelium with a very tight permeability barrier to prevent paracellular fluxes of urine. This barrier is formed by non-regulated tight junctions between facet cells, the uppermost cell layer easily identified by their large nuclei (asterisks). Arrows in b and d indicate the tight junctions that can be visualized by their occludin content (b, red), but are devoid of Kir6.1 protein (a). Bar, 50 µm.





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