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Figure 3


Fig. 3. Tubule constrictions are encircled by a Dnm1p spiral (or rings) that usually contain 100 to 500 Dnm1p molecules. (A-C) Confocal image of a wt cell expressing mtDsRed (A), Dnm1p-GFP (B) and the overlay of both images together with a bright field image (C). The arrow points to a tubule constriction coinciding with a Dnm1p-GFP assembly. Bar 1 µm. (D) Fluorescence intensity profiles in the GFP and DsRed channel (added up in the y-direction) within the rectangle shown in C at a tubule constriction. (E,F) Fluorescence intensity distributions of GFP-VLPs (gray) and Dnm1p-GFP spirals (green) normalized to the average fluorescence intensity of a single GFP-VLP in wt (E) and caf4{Delta} cells (F). Note the differing y-axes values in E and F. All Dnm1p-GFP spiral-like structures were scrutinized manually. One GFP-VLP contains 120 GFP molecules.





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