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Figure 5


Fig. 5. Polar localization of mitochondrial Dnm1p-GFP clusters is maintained in the absence of F-actin. (A) The actin cytoskeleton partly colocalizes with the mitochondrial network in wt cells. (Left panel) Actin cytoskeleton stained with phalloidin-conjugated Alexa Fluor-546. (Middle panel) GFP-labelled mitochondria. (Right panel) Corresponding bright-field image. (B) Bar graphs show the polarity of the mitochondria-associated Dnm1p-GFP clusters in wt cells treated with the actin depolymerizing drug Latrunculin A for 40 minutes (LatA 40') or without (untreated). A majority of the clusters point to the cell cortex (out). Right: Bright-field and fluorescence image of a representative wt cell treated with Latrunculin A for 40 minutes expressing Dnm1p-GFP and mtDsRed. Bars, 1 µm.





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