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Fig. 6. Polarized mitochondria-associated Dnm1p-GFP clusters appear to be physically attached to the cell cortex. Shown is a wt cell expressing Dnm1p-GFP and mtDsRed after formaldehyde fixation. Displayed is an add-up of confocal sections of the two fluorescence channels overlaid with a bright-field image. After formaldehyde fixation the mitochondrial tubules retract occasionally from the cell cortex, as shown in this image. Arrowheads point to mitochondria-associated Dnm1p-GFP clusters that appear to be attached to the cell cortex. The arrow points to a Dnm1p-GFP cluster that stayed at the cell cortex and might have been torn off from the mitochondrial tubule. Bar, 1 µm.