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Figure 1


Fig. 1. (A) Diagram of domains within complete coding sequence of CrKCBP. CrKCBP contains a myosin tail homology domain (MyTH4), a band 4.1 domain (B41), present in a number of proteins that interface between the plasma membrane and cytoskeleton, a coiled-coil neck region (CC), a kinesin motor domain (KMD), and a calmodulin-binding domain (CBD). (B) RNA blot. 10 µg of poly(A)+ selected RNA prepared from control cells (lane 1), 45 minutes after deflagellation (lane 2) or 45 minutes after induction of flagella resorption (lane 3) were loaded into each lane. The blot was probed with a DNA fragment at the 5' end of the CrKCBP coding sequence (labeled Probe in A) as well as the S14 gene encoding ribosomal S14 protein to serve as a loading control. The band of approximately 1 kb represents the S14 transcript. The band at approximately 4.4 kb represents the CrKCBP transcript. This transcript increases in abundance following deflagellation and decreases slightly in abundance during flagellar resorption.





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