|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. Immunoblot. Flagella (Fl) were isolated from wild-type cells and demembranated as described in Materials and Methods. The resulting membrane and matrix fraction (M) and axonemes (Ax) were isolated by centrifugation. Axonemes were divided and extracted with either 0.6 M NaCl (NaCl), 1 mM ATP (1ATP), or 5 mM ATP (5ATP) to produce extracted axonemes (EA) and axonemal extracts (E). The resulting extracted axonemes and extracts were separated by centrifugation and extracted axonemes were resuspended in an equal volume of buffer. For immunoblot analysis, 25 µl of each sample were loaded in each lane and anti-CrKCBP antibodies were used as a probe. These antibodies recognize a single band of approximately 140 kDa. This protein is extracted from axonemes under conditions of high salt and ATP. CrKCBP appears to be of higher molecular mass in flagella because of the large quantity of flagellar membrane in this sample, which interferes with protein migration.
|
