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Figure 3


Fig. 3. Localization of CrKCBP by indirect immunofluorescence. Axonemes were isolated as described in the Materials and Methods. Samples in the right column were extracted with 5 mM ATP prior to processing for immunofluorescence. The axonemes were double labeled for tubulin (top row, Texas Red secondary antibody), and CrKCBP (middle row, Alexa Fluor 488 secondary antibody). The merged images are shown in the bottom row where white indicates co-localization of tubulin with CrKCBP. CrKCBP localizes along the length of the axoneme and is extracted upon exposure to 5 mM ATP.





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