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Figure 5


Fig. 5. Indirect immunofluorescence localization of CrKCBP during interphase. (A) Localization of tubulin (Texas Red secondary antibody) and CrKCBP (Alexa Fluor 488 secondary antibody) in Chlamydomonas interphase cells. Areas of co-localization appear white in the merged image. Lower panels are enlargements of the region with the greatest CrKCBP staining. The significant fraction of CrKCBP localizes near the base of the flagellar microtubules. Bars, top row, 10 µm; bottom row, 1 µm. (B) Immunoblot. Flagellar basal body complexes (FBB) and flagella (FL) were prepared from equal numbers of cells and equal amounts were loaded onto the gel. The resulting blot was probed with antibodies against CrKCBP as well as PF20, a known axonemal component.





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