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Fig. 7. Cellular localization of CrKCBP during mitosis as shown by indirect immunofluorescence. Cells were triple labeled with anti-centrin (Alexa Fluor 594 secondary antibodies), anti-CrKCBP (Alexa Fluor 488 secondary antibodies) and anti-tubulin (Alexa Fluor 350 secondary antibodies) antibodies. As the cells progress through mitosis the CrKCBP staining appears concentrated at the duplicated microtubule organizing centers. However, as the cells progress to cytokinesis CrKCBP appears to localize to the phycoplast. Bar, top row, 10 µm; bottom row, 1 µm.