spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. (A) Quantitative real-time PCR to assess M-CAM expression in fetal tissue. The different amplification curves demonstrate diverse M-CAM cDNA content at the three time points. Mb, myoblasts; E, L Mt, early and late myotubes. GAPDH was used as internal control. Each PCR reaction was performed in duplicate. (B) Downregulation of M-CAM RNA during myoblast fusion as determined by real-time quantitative PCR analysis. The fold change value (y axis) in the late myotubes sample was arbitrarily set as 1 and the fold change values in early myotubes and myoblasts were plotted accordingly. (C) Agarose gel of PCR products after amplification. Sequence analysis confirmed that the band corresponds to M-CAM. M, molecular size markers. Lane 1, myoblasts; lane 3, early myotubes; lane 5, late myotubes; lanes 2, 4, and 6 are water controls for each reaction.





Right arrow Return to article