spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Evaluation of myogenic potential of cells fractionated by M-CAM and CD31 expression. (A) FACS analysis of negative control used to set up the gates for cell fractionation. 99.7% of human fetal cells are double negative. (B) M-CAM and CD31 expression in human fetal primary muscle cells. 51.5% of cells are M-CAM-positive, 0.6% are double positive, 1.28% are CD31-positive and 46.6% are double negative. Cells were fractionated according to the displayed gates. (C-N) Brightfield and immunofluorescence images of sorted human cell populations stained for the myogenic marker desmin (red) and myosin heavy chain (MHC, green) after differentiation. Nuclei are stained in blue with DAPI. (C,D,E) M-CAM+CD31- cells; (F,G,H) M-CAM+CD31+ cells; (I,J,K) are M-CAM-CD31+; (L,M,N) are M-CAM-CD31-. Magnification, 100x. (O) Percentage of desmin-positive cells in fractionated cell populations as determined by counts performed in ten random fields of view. (P) Fusion index of the fractionated cell populations confirming that cells expressing M-CAM are highly myogenic.





Right arrow Return to article