spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Stable overexpression of human Cdt1 in Rat-1 cells activates the ATM-Chk2 DNA damage checkpoint pathway without detectable rereplication. (A) Whole-cell lysates prepared from Rat-1 clones overexpressing wild-type T7-Cdt1 (WA4, WB1, WB3 and WB4) or T7-Cdt1 Cy (CA2, CA3, CB3 and CB4) and from parental Rat-1 cells were immunoblotted. Hydroxyurea (HU)-treated Rat-1 cells were analyzed similarly. (B) Cell-cycle analysis of the Rat-1 clones. Exponentially growing clones were analyzed at 2 months post-infection. (C) Growth rates of the Rat-1 clones. (D) Whole cell extracts, Triton X-100-extractable fractions, and nuclear chromatin/matrix fractions were prepared from WB4 cells and immunoblotted as described in Fig. 2E.





Right arrow Return to article