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Figure 7


Fig. 7. Deregulated Cdt1 leads to chromosomal instability in normal human fibroblasts. (A) HFF2 cells were infected with the T7-Cdt1 retroviruses as in Fig. 6, and subjected to karyotypic analysis at 2 months post-infection. We performed four independent infection experiments, and chromosome distributions included the analysis of approximately 50 metaphase spreads for each experiment. The sum of results from the four experiments is shown. For parental HFF2 cells, the analysis was performed on 100 metaphase spreads. (B) Representative results of more detailed karyotypic analysis. (Top panel) Shown are normal diploid with 46 chromosomes observed in control vector-infected cells. (Middle panels) Shown are 48 chromosomes with trisomy 5 and trisomy 10 (left) and 47 chromosomes with trisomy 10 (right) observed with wt Cdt1. (Bottom panels) Shown are 45 chromosomes with Y deficiency (left) and 48 chromosomes with trisomy 7 and trisomy 10 (right) observed with Cdt1 Cy.





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