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Figure 1


Fig. 1. The AP-2 adaptor complex is necessary for the norpA-induced endocytosis of stable rhodopsin-arrestin complexes. All panels show cross sections (0.75 µm) from a frozen Drosophila retina stained with both arrestin and rhodopsin antibodies followed by fluorescein- and rhodamine-labeled secondary antibodies respectively. (A-C) Wild-type, (D-F) norpA and (G-I) norpA;{alpha}-adaptin. The flies were dark-reared and then treated with constant room light for 24 hours prior to fixation. Dissected eyes were fixed and sectioned as described in Materials and Methods. A, D and G were stained with arrestin-specific antibodies, B, E and H were stained with rhodopsin-specific antibodies, C, F and I are merged images.





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