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Figure 5


Fig. 5. Phenotype of neonatally BrdU-labelled cells in 60-day-old rats. Merged confocal microscope images of immunoreactivity for BrdU (in green), and NeuN (in red) (A,B) and for BrdU (in green), and GFAP (in red) (C) in 40 µm thick sections of cerebellum of 60-day-old animals; colocalization appears yellow. (A) Control, (B,C) P1-P3 L-NAME-treated rats. These animals received on P3 a BrdU injection (100 µg/g body weight) after 3 days administration of L-NAME or vehicle and were sacrificed at 60 days of age. (D) Number of BrdU and NeuN (white) or BrdU and GFAP (hatched) labelled cells expressed as a percentage of total number of BrdU-labelled cells. The black part of the bars represents the percentage of cells that were of indeterminate phenotype. Values are the mean ± s.e.m. of three experiments.





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