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Figure 2


Fig. 2. Stimulation of S. cerevisiae acetic-acid-induced apoptosis by expression of mammalian PKC-{alpha}, -{epsilon} or -{zeta} but not PKC-{delta} isoform is associated with significant ROS production. ROS production by mitochondria was detected by flow cytometry, using MitoTracker Red CM-H2XRos. Overlays of red fluorescence histograms were obtained with yeast treated with 0 and 300 mM acetic acid: black fill, yeast transformed with the empty vector (YEplac181 or YEp51); light-grey line, yeast expressing PKC-{alpha}, -{delta}, -{epsilon} or -{zeta}. Cells treated in the absence of acetic acid (0 mM) were used as a negative control. Data represent one of two independent experiments.





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