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Figure 1


Fig. 1. Microtubule dynamics during NE breakdown. (A-H) Gradual appearance of GFP-MAP4-labelled microtubules around the NE before its disintegration. Note the GFP-MAP4-labelled microtubules penetrate inside the NE before spindle initiation (image C-F). (I-L) Dynamics of GFP-AtEB1-labelled microtubule plus ends during NE breakdown. Microtubule plus ends are in green and FM4-64-labelled endocytic vesicles are in red. White arrows indicate plus ends of microtubules penetrating inside NE from the polar region; white arrowheads indicate plus ends of microtubules penetrating inside NE in a direction perpendicular to the confocal section; the yellow arrow indicates integrity of the NE. Note the appearance of GFP-AtEB1 inside the NE by microtubule penetrations and the continuity of NE integrity as revealed by the persistence of endocytic vesicles outside the nuclear area until significant NE breakdown. Bars, 5 µm; time is indicated in seconds.





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