spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Growth polarity and growth speed of spindle microtubules during metaphase. (A) `xt' kymograph (horizontal line projection) of GFP-AtEB1-labelled spindle microtubules corresponding to the equatorial position in B (see corresponding arrows and arrowheads showing hardly any lateral movement of the areas containing chromosomes and those without chromosomes; yellow lines in A exactly match the areas shown by red lines in B, except that the yellow lines represent horizontal projection and the red lines vertical projection). (B) Metaphase spindle microtubules labelled with GFP-AtEB1. This is an initial image of a time-lapse series shown in Movie 2 from supplementary material. Panels C-H correspond to chromosomal areas and panels I-N correspond to overlapping microtubule areas without chromosomes near the equator. (C,D) `yt' kymograph obtained from the left line drawn in B. (I,J) `yt' kymograph obtained from the right line drawn in B. Rectangles in D and J indicate the areas taken for the Fourier analysis. The Fourier transformed images of the combined kymographs of all near chromosomal areas and of all overlapping microtubule areas without chromosomes in the entire spindle are given in E and K, respectively. All areas and individual kymographs are shown separately in supplementary material Fig. S2. The areas selected for obtaining the microtubule growth speeds from the Fourier images are shown in F and L. The specially developed image processing program Object-Image was used, which allows detector regions to be defined based on a local, polar co-ordinate system. A ring sector region of ±90 degrees was marked (F,L), with clockwise angles as positive and the x-axis corresponding to zero degrees for averaging the pixels with the same angle and yielding an angular intensity profile (G). (G,M) Angular intensity distribution of Fourier graph (F) and (L). (H,N) Graph with reciprocal x-axis (from graph G and M) showing microtubule growth speed distribution in spindle areas. Bar, 7 µm.





Right arrow Return to article