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Fig. 6. Microtubule polarity and growth speed along the spindle-axis. Kymograph obtained from a line drawn over a chromosomal area (A) and overlapping microtubule area without chromosome (B) displaying growth polarity of GFP-AtEB1-labelled spindle microtubules. Fourier graphs in C are obtained from four parts of the combined kymograph representing the entire spindle area, which is assembled from various kymographs obtained by drawing subsequent lines on whole spindle area. (D) Angular intensity distribution and growth speed distribution of the Fourier graphs from C.